Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats

Fischer, Melina GND; Freuling, Conrad Martin GND; Müller, Thomas GND; Schatz, Juliane GND; Bruun Rassmussen, T.; Chriel, M.; Balkema-Buschmann, Anne GND; Beer, Martin GND; Hoffmann, Bernd GND

Background In the frame of active lyssavirus surveillance in bats, oropharyngeal swabs from German (N = 2297) and Danish (N = 134) insectivorous bats were investigated using a newly developed generic pan-lyssavirus real-time reverse transcriptase PCR (RT-qPCR). Findings In total, 15 RT-qPCR positive swabs were detected. Remarkably, sequencing of positive samples did not confirm the presence of bat associated lyssaviruses but revealed nine distinct novel rhabdovirus-related sequences. Conclusions Several novel rhabdovirus-related sequences were detected both in German and Danish insectivorous bats. The results also prove that the novel generic pan-lyssavirus RT-qPCR offers a very broad detection range that allows the collection of further valuable data concerning the broad and complex diversity within the family Rhabdoviridae.

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Fischer, Melina / Freuling, Conrad Martin / Müller, Thomas / et al: Identification of rhabdoviral sequences in oropharyngeal swabs from German and Danish bats. 2014.

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